Lumigen APS-5
Lumigen® APS-5 utilizes a unique technology for the chemiluminescent detection of alkaline phosphatase conjugates and provides superior sensitivity and ease of use. Reaction of the acridan substrate with an AP label produces sustained high-intensity chemiluminescence. Lumigen APS-5 is ideally suited for solution assays of phosphatase activity and for phosphatase-linked immunoassays.An Applications Note illustrating the sensitivity of Lumigen APS-5 in the detection of alkaline phosphatase on a microplate luminometer is available from Turner BioSystems.
Lumigen APS-5 in Homogeneous Alkaline Phosphatase Assays
- Excellent sensitivity -- less than 10-19 moles AP can be measured.
- Rapid develpment of peak signal reduces assay time and increases throughput.
- Linear calibration curves with slopes of log-log plots equal to 1.0 -- one order of magnitude more enzyme yields one order of magnitude more luminescence.
- Sustained luminescence -- timing is less critical. Light intensities can be read at any time to produce linear calibration curves.
- Analytical results are insensitive to temperature from 22°C to 35°C reducing the need for precise temperature control.
Rapid Chemiluminescent Immunoassay
A sandwich immunoassay for thyroid stimulating hormone (TSH) performed with an Immulite TSH Third Generation TSH Assay kit yielded a linear plot from 0.003 mIU/L to 75 mIU/L with excellent assay precision. Lumigen APS-5 was substituted for the normal dioxetane substrate, Lumigen PPD.
Western Blotting
The image below from a western blot analysis of human transferrin was made after a 10 minute incubation of the PVDF membrane with Lumigen APS-5 with a 1 minute exposure to X-OMAT film. Equivalent results were obtained using nitrocellulose membranes. No other chemiluminescent reagent allows complete interchangeability of blotting membrane.
Lumigen APS-5 Ordering Information
| Catalog No | Volume | Price (USD) |
| AP5-101 | 100mL | $270  |
Prices shown are effective 1-Jan-09.
Prices shown are subject to change.
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