Hydrogen Peroxide Detection

The measurement of hydrogen peroxide produced enzymatically is of growing interest for the study of a variety of biological functions and the analysis of physiological effects of oxidative stress. Lumigen® HyPerBlu® is a novel substrate for the direct chemiluminescent detection of hydrogen peroxide. Reaction of the substrate with hydrogen peroxide rapidly generates sustained high intensity luminescence for maximum detection sensitivity in solution assays. Couple with oxidases, HyPerBlu allows one step indirect detection of oxidases or their substrates.