ELISA AP Substrate
Lumigen APS-5 is a proprietary acridan based chemiluminescent substrate for ELISA detection of alkaline phosphatase (AP) conjugated molecules. The unique acridan chemistry of Lumigen APS-5 provides temperature insensitive light output and more rapid peak intensity as compared to the dioxetane based AP substrates, Lumi-Phos 530 and Lumi-Phos Plus.
- Excellent sensitivity - low picogram to femtogram
- Rapid peak intensity - reduced assay time and increased throughput
- Temperature insensitive - analytical results are insensitive to temperatures from 22° C - 35° C reducing the need for precise temperature control
- Sustained luminescence - timing is less critical with light intensities able to be read at any time to produce linear calibration curves
Rapid Peak Intensity
The light-output peak intensity of Lumigen APS-5 reaches sustained maximum within seconds of substrate addition. Light emission is maximal at 450 nm.
Lumigen APS-5 is available in 100 mL and 1 Liter volumes. Visit the Lumigen store to purchase on-line.
Detection Technology
Download
Data Sheet:
Application Instructions:
Handling Instructions:
Product Specifications
Enzyme:
Alkaline Phosphatase (AP)
Application:
ELISA
Sensitivity:
Low picogram to femtogram
Storage Conditions:
2-8° C; Store in amber bottle to protect from light
Stability:
1 year stored at 2-8° C
Working Solution:
Ready to use solution
Ordering Information
Catalog Number:
AP5-101
AP5-1000
Description:
Lumigen APS-5 (100 mL)
Lumigen APS-5 (1 L)